Review



primescripttm rtreagent kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa primescripttm rtreagent kit
    Primescripttm Rtreagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rtreagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm41546958-87-13-17
    Average 99 stars, based on 15514 article reviews
    primescripttm rtreagent kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots
    Article Snippet: .. The synthesis of cDNA was performed using the PrimeScriptTM RTreagent Kit and gDNA Eraser (Perfect Real Time) Kit (TaKaRa). cDNA synthesized by reverse transcription was used as PCR template and reference gene Triticum aestivum glyceraldehyde-3-phosphatedehydrogenase (TaGAPDH) was used as a control. .. Related gene sequences of wheat obtained from NCBI, and gene-speci c primers (Table 1) were designed by Primer Premier 5.0 software, and then the primers were synthesized by Shanghai ShengGong Bioengineering Co., LTD.

    Reverse Transcription:

    Article Title: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots
    Article Snippet: .. The synthesis of cDNA was performed using the PrimeScriptTM RTreagent Kit and gDNA Eraser (Perfect Real Time) Kit (TaKaRa). cDNA synthesized by reverse transcription was used as PCR template and reference gene Triticum aestivum glyceraldehyde-3-phosphatedehydrogenase (TaGAPDH) was used as a control. .. Related gene sequences of wheat obtained from NCBI, and gene-speci c primers (Table 1) were designed by Primer Premier 5.0 software, and then the primers were synthesized by Shanghai ShengGong Bioengineering Co., LTD.

    Article Title: A New Rat Model of Sacral Cord Injury Producing a Neurogenic Bladder and Its Functional and Mechanistic Studies
    Article Snippet: .. Total RNA was extracted using Trizol reagent (InvitrogenTM, Waltham, MA, USA, Cat.No:15596018CN) and then reverse-transcribed into cDNA with the PrimeScriptTM RTreagent Kit (Takara Biotech, Kusatsu, Japan, Cat. No: RR037A). .. Quantitative realtime PCR (qRT-PCR) was conducted using specific primers and PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™, Waltham, MA, USA, Cat. No: A25742) on a C1000 Touch Thermal Cycler equipped with a CFX96 real-time system.

    Article Title: Ubiquitin-specific peptidase 10 attenuates the ferroptosis to promote thyroid cancer malignancy by facilitating GPX4 via elevating SIRT6.
    Article Snippet: Funding information The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine; the Shanghai Pudong New Area key specialty project, Grant/Award Number: PWZzk2022-12; the Pudong New Area Science and Technology Development Fund, Grant/Award Number: PKJ2020-Y18; the Budget Project of Shanghai University of Traditional Chinese Medicine, Grant/Award Number: 2021LK057; the Talent Training Program (the Big Dipper) of Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Grant/Award Number: BDX2022-04 Abstract Purpose: Ubiquitin-specific peptidase 10 (USP10) has been found to have oncogenic activity in several human tumors.. This study first revealed the exact function of USP10 on the progression of thyroid cancer (THCA) by researching its effect on the ferroptosis.. Methods: USP10 expression in THCA patients was analyzed by online data analysis and in 75 THCA cases was scrutinized by real-time quantitative reverse transcription-polymerase chain reaction and Western blot.

    Article Title: Dihydrotestosterone binding to the androgen receptor inhibits apoptosis in ovine undifferentiated granulosa cells via AMH signaling.
    Article Snippet: Although dihydrotestosterone (DHT), androgen receptor (AR), and anti-Müllerian hormone (AMH) play key roles in ovarian function, their precise contributions and synergistic roles in mammalian follicular development remain unclear.. In this study, we first evaluated whether ovine granulosa cells (GCs) undergo differentiation during vitro culture and then determined methods for inhibiting differentiation.. Following the culture of GCs with DHT (10 mol/L) and the AR antagonist enzalutamide (ENZ) (10 mol/L) separately or in combination for 48 h, an apoptosis assay revealed that the interaction between DHT and AR decreases sheep GC apoptosis.

    Polymerase Chain Reaction:

    Article Title: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots
    Article Snippet: .. The synthesis of cDNA was performed using the PrimeScriptTM RTreagent Kit and gDNA Eraser (Perfect Real Time) Kit (TaKaRa). cDNA synthesized by reverse transcription was used as PCR template and reference gene Triticum aestivum glyceraldehyde-3-phosphatedehydrogenase (TaGAPDH) was used as a control. .. Related gene sequences of wheat obtained from NCBI, and gene-speci c primers (Table 1) were designed by Primer Premier 5.0 software, and then the primers were synthesized by Shanghai ShengGong Bioengineering Co., LTD.

    Article Title: Humid heat environment causes anxiety-like disorder via impairing gut microbiota and bile acid metabolism in mice.
    Article Snippet: Images were captured with a confocal microscope (Zeiss, Germany) and ImageJ2 microscope (Zeiss, Germany). .. Total RNA was extracted from the mouse prefrontal cortex using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and the RNA concentration was determined using a Nanodrop 2000c spectrophotometer (Thermo, USA). cDNA was synthesised from 1μg of RNA using a PrimeScriptTM RTReagent Kit with gDNA Eraser (RR047A, Takara, Japan), and the PCR mixture was prepared with TaKaRa TB GreenTM Premix Ex TaqTM II (RR820A, Takara, Japan). .. The cDNA in the mixtures was subjected to PCR using a realtime PCR system (Bio-Rad, USA).

    Control:

    Article Title: Lignin metabolism involved in zinc-alleviating effects on iron-induced phytotoxicity in wheat roots
    Article Snippet: .. The synthesis of cDNA was performed using the PrimeScriptTM RTreagent Kit and gDNA Eraser (Perfect Real Time) Kit (TaKaRa). cDNA synthesized by reverse transcription was used as PCR template and reference gene Triticum aestivum glyceraldehyde-3-phosphatedehydrogenase (TaGAPDH) was used as a control. .. Related gene sequences of wheat obtained from NCBI, and gene-speci c primers (Table 1) were designed by Primer Premier 5.0 software, and then the primers were synthesized by Shanghai ShengGong Bioengineering Co., LTD.

    Gene Expression:

    Article Title: Dihydrotestosterone binding to the androgen receptor inhibits apoptosis in ovine undifferentiated granulosa cells via AMH signaling.
    Article Snippet: Although dihydrotestosterone (DHT), androgen receptor (AR), and anti-Müllerian hormone (AMH) play key roles in ovarian function, their precise contributions and synergistic roles in mammalian follicular development remain unclear.. In this study, we first evaluated whether ovine granulosa cells (GCs) undergo differentiation during vitro culture and then determined methods for inhibiting differentiation.. Following the culture of GCs with DHT (10 mol/L) and the AR antagonist enzalutamide (ENZ) (10 mol/L) separately or in combination for 48 h, an apoptosis assay revealed that the interaction between DHT and AR decreases sheep GC apoptosis.

    other:

    Article Title: Identifying lncRNAs and mRNAs related to survival of NSCLC based on bioinformatic analysis and machine learning
    Article Snippet: The total RNA was reverse transcribed into the cDNA template by using the PrimeScript RTreagent kit (Takara, Beijing, China).

    Concentration Assay:

    Article Title: Humid heat environment causes anxiety-like disorder via impairing gut microbiota and bile acid metabolism in mice.
    Article Snippet: Images were captured with a confocal microscope (Zeiss, Germany) and ImageJ2 microscope (Zeiss, Germany). .. Total RNA was extracted from the mouse prefrontal cortex using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and the RNA concentration was determined using a Nanodrop 2000c spectrophotometer (Thermo, USA). cDNA was synthesised from 1μg of RNA using a PrimeScriptTM RTReagent Kit with gDNA Eraser (RR047A, Takara, Japan), and the PCR mixture was prepared with TaKaRa TB GreenTM Premix Ex TaqTM II (RR820A, Takara, Japan). .. The cDNA in the mixtures was subjected to PCR using a realtime PCR system (Bio-Rad, USA).

    Spectrophotometry:

    Article Title: Humid heat environment causes anxiety-like disorder via impairing gut microbiota and bile acid metabolism in mice.
    Article Snippet: Images were captured with a confocal microscope (Zeiss, Germany) and ImageJ2 microscope (Zeiss, Germany). .. Total RNA was extracted from the mouse prefrontal cortex using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and the RNA concentration was determined using a Nanodrop 2000c spectrophotometer (Thermo, USA). cDNA was synthesised from 1μg of RNA using a PrimeScriptTM RTReagent Kit with gDNA Eraser (RR047A, Takara, Japan), and the PCR mixture was prepared with TaKaRa TB GreenTM Premix Ex TaqTM II (RR820A, Takara, Japan). .. The cDNA in the mixtures was subjected to PCR using a realtime PCR system (Bio-Rad, USA).



    Similar Products

    99
    TaKaRa primescripttm rtreagent kit
    Primescripttm Rtreagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rtreagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm41546958-87-13-17
    Average 99 stars, based on 1 article reviews
    primescripttm rtreagent kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    TaKaRa primescripttm rtreagent kit with gdnaeraser
    Primescripttm Rtreagent Kit With Gdnaeraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rtreagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pm41350926-237-7-13
    Average 99 stars, based on 1 article reviews
    primescripttm rtreagent kit with gdnaeraser - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results